EFFECT OF STORAGE CONDITIONS OF SPORES OF PENICILLIUM DIGITATUM ON ITS PARAMETERS OF GROWTH




María Luisa Carrillo Inungaray (CV)
maluisa@uaslp.mx
Sasi Elibeth Martini Morales (CV)
Sasi.martini@uaslp.mx
Víctor José Robles Olvera (CV)
vrobles@itver.edu.mx
Universidad Autónoma de San Luis Potosí


ABSTRACT
The objective of this work was determine the effect of temperature (0 and -9°C) and time (0, 7, 14 and 21 days) of storage, on the parameters of growth, (lag time and growth rate) of one spore suspension of Penicillium digitatum. Experimental conditions were established by one factorial full design. The growth was realized in dextrose Sabouraud agar and the diameter of the colony was measured daily. The parameters of growth were calculated by fit of the growth curves with the Baranyi equation using the DMFit software. The results showed that the lag time first increased with the duration of storage in low temperature and then decreased as the duration of storage (p< .05), while the growth rate only was influenced by the time of storage and not by the temperature.

Keywords
Penicillium digitatum, spore, lag time, growth rate.

RESUMEN
El objetivo de este trabajo fue determinar el efecto el efecto de la temperatura (0 y -9°C) y tiempo (0, 7, 14 y 21 días) de almacenamiento, sobre los parámetros del crecimiento (tiempo de latencia y velocidad de crecimiento) de una suspensión de esporas de Penicillium digitatum. Las condiciones experimentales fueron establecidas por un diseño factorial completo. El crecimiento fue realizado en agar dextrosa Sabouraud y el diámetro de las colonias fue medido diariamente. Los parámetros de crecimiento fueron calculados por el ajuste de las curvas del crecimiento con la ecuación de Baranyi usando el programa DMFit. Los resultados demostraron que el tiempo de latencia inicialmente aumenta con la duración del almacenamiento en baja temperatura y posteriormente disminuye conforme aumenta el tiempo de almacenamiento (p< .05), mientras que la velocidad de crecimiento solamente fue influenciada por el tiempo y no por la temperatura de almacenamiento.

PALABRAS CLAVE
Penicillium digitatum, esporas, tiempo de latencia, velocidad de crecimiento.

 

INTRODUCTION
Food microbiologists know that spores are one low activity metabolic form resistant to adverse factors such as low temperature, for this some researchers realize one reactivation step by avoiding lag time prolonged while another esteem that such effect not exist and realize the kinetics directly of the refrigerated spore suspension.
Some researchers affirm that the damaged cells, one time that recover them generate the same subpopulations that those cells not damaged (Baranyi and Roberts, 1993), other researchers have confirmed that temperature historic, yet without verify cell damage, affect the behavior of cells microbial to inoculate them in culture media (McKellar and Knigth, 2000). Some works have been realized in bacteria (Jackson and Wodbine, 1963) in which have observed one increased in the lag time with the stress duration but they studied only one stress temperature. Bréand et al. (1997) observed that for a fixed stress temperature, the lag time first increased with the stress duration and then decreased as the stress duration increased. There is little information about the effect of stress temperature on lag time of bacteria, and less on lag time and growth rate of filamentous fungi.
Most of studies of fungi growth require one spore suspension to inoculate the culture media or foods. Some investigators prepare such suspension culturing the strains on potato-dextrose agar slants for 10 days at 25°C and harvest the spores with 10 mL of 0.1% Tween 80 and used it the same day (López-Malo et al., 1998), or prepared of one culture of 6 days at 20°C and harvesting the spore suspension in deionized water containing 0.2 % Tween 20 (Chardonnet et al., 2002) while another do it of one culture of 5 days to 25°C  (Stiles Battey et al., 2001).
From the food predictive microbiology viewpoint, the way in that growth rate data are obtained is important since the study of fungal growth is realized trough the growth kinetic based in compilation of growth data and then to generate mathematical models capable to predict the growth parameters of molds in different conditions. Thus the aim of this work was to study the response of Penicillium digitatum when the spore suspension employed for calculate its parameters was storage in stress conditions.

MATERIALS AND METHODS
Spores suspension
In this study was used Penicillium digitatum isolated of citrus fruits and identified accorded to Samson et al. (1995/d) and was grown on dextrose Sabouraud agar for 7 days at 25°C, the time necessary to obtain cultures with sufficient spores, which were harvested with 10 mL of 0.1% Tween 80 (Merck) solution sterilized by membrane (0.45 m) filtration. The spore suspension was adjusted with the same solution to give a final spore concentration of 106 spore/mL. The suspension was distributed in three portions and were treated according to full factorial design: one of them was employed immediately after its preparation and was used as reference, the other two portions were storage to 0 and -9°C for 7, 14 and 21 days.

Inoculation
Plates of dextrose Sabouraud agar with water activity 0.990 and pH 5.0 were inoculated directly with 2 L of each one portions and too were revitalized in agar dextrose Sabouraud slants to 25°C for 7 days and prepared the spore suspension again, proceeding after to inoculate the culture media. All experiments were carried out with at least three separate replicate Petri plates per treatment and collocated in plastic box and were incubated to 30°C for 30 days.

Growth measurement
The plates inoculated were observed every day to observe growth and each 24 hours the diameter of colonies was measured with one ruler. The diameter of colonies was plotted against time and was obtained growth curves of each condition which were adjusted with the Baranyi equation using the software DMFit, obtaining thus the parameter growth of mold in each condition.

RESULTS AND DISCUSSION
Table 1 show the parameters of growth obtained in each studied condition and the analyses of the results showed one significant effect (p < 0.05) of storage time, and activation of spores on the lag time (Fig. 1) was observed that in accordance with increase the time storage in stress condition the effect decreased. Similar results were obtained for Bréand et al. (1999) for lag time in bacterial culture, although not explain the phenomena. Effect of temperature was not significant for lag time.

Table 1. Parameters growth – lag time (l) and growth rate (m) of P. digitatum storage to 0° and -9°C, not activated (NA) and activated (A) in dextrose Sabouraud agar.

Tabla 1. Parámetros de crecimiento –tiempo de latencia (l) y velocidad de crecimiento (m) de P. digitatum almacenado a 0° y -9°C, no activada (NA) y activada (A) en agar dextrosa Sabouraud.

Parámetros de crecimiento
Respect to growth rate, was observed only effect of time (Fig 2), temperature and activation not were significant. However such effect, although is statistically significant, in practice is not, since to make round the growth rate is equal in all conditions. This results are coherent with the concepts and definitions of lag time and growth, due to that is waited that the low metabolism present in the spores and induced by storage conditions, affect only to lag time and that one time that start the development, the growth rate permanence invariable (Baranyi and Roberts, 1993).
This results doing suppose that despite the low activity inherent to the spores, these put in march one physiological mechanism by adapt to stress, and is manifested when the spores present lag time similar to those spores obtained recently.

CONCLUSIONS
The results shown that stress to low temperature has effect on lag time of P. digitatum, which spores were storage in low temperature, however, such effect decreased when the duration of stress was increased, while the growth rate is affected only by effect the time of storage and not by the temperature.

REFERENCES
Baranyi J.; Roberts, T. A.; McClure, P. 1993. A non-autonomous differential equation to model bacterial growth. Food Microbiology 10, 43-59.

Chardonnet, C. O.; Sams, C. E.; Conway, W. S.; draughon, F. A.; Mount, J. R. 2002. Osmotic dehydration of apple slices with CaCl2 and sucrose limits decay caused by Penicillium expansum, Colletotrichum acutatum and Botrytis cinerea and does not promote Listeria monocytogenes or total aerobic population growth. Journal of Food Protection 65, 172-177.

Bréand, S.; Fardel, G.; Flandrois, J. P.; Rosso, L.; Tomassone, R. 1997. A model describing the relationship between lag time and mild temperature increase duration. International Journal of Food Microbiology 38, 157-167.

Bréand, S.; Fardel, G.; Flandrois, J. P.; Rosso, L.; Tomassone, R. 1999. A model describing the relationship between regrowth lag time and mild temperature increase for Listeria monocytogenes. International Journal of Food Microbiology 46, 251-261.

Jackson, H.; Woodbine, M. 1963. The effect of sublethal heat treatment on the growth of Staphylococcus aureus. Journal of Applied Bacteriology 26, 152-158.
López-Malo, A.; Alzamora, S. M.; Argaiz, A. 1998. Vanillin and pH sinergystic effects on mold growth. Journal of Food Science 63, 143-146.

McKellar, R. C.; Knight, K. 2000. A combined discrete-continuous model describing the lag phase of Listeria monocytogenes. International Journal of Food Microbiology 54, 171-180.

Samson, R. A.; Hoekstra, E, S.; Frisvad, J. C.; Filtenborg, O. 1995. Introduction to Foodborne Fungi, pp 322.  En: Centraalbureau Voor Schimmelcultures (Ed), 4th ed. Baarn, the Netherlands,

Stiles Battey, A.; Duffy, S.; Schaffner, D. W. 2001. Modelling mould spoilage in cold-filled ready-to-drink beverages by Aspergillus niger and Penicillium spinulosum. Food Microbiology 18, 521-529.

Fecha de recepción: 2 de octubre de 2013
Fecha de aceptación: 3 diciembre de 2013
Fecha de publicación: diciembre 2013


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